Tribioscience D-3-Hydroxybutyrate dehydrogenase, Enzyme Activity (TBP0027)

Research Use Only. Not intended for human use.

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 100U/mg-solid or more

Contaminants: Malate dehydrogenase ≤2.0×10⁻³%

Lactate dehydrogenase ≤2.0×10⁻³%

NADH oxidase ≤2.0×10⁻³%

Stabilizers: Sucrose, mannitol, BSA

Properties

Stability: Stable at -20°C for at least Two years

Molecular weight: approx. 130,000 (by gel filtration)

Isoelectric point: 5.6±0.1

Michaelis constants: 4.2×10⁻⁴M (25°C, pH8.3), 7.0×10⁻⁴M(37°C, pH8.3)(D-3-Hydroxybutyrate)

4.9×10⁻⁵M (25°C, pH8.3), 7.2×10⁻⁵M (37°C, pH8.3)(NAD⁺)

8.1×10⁻⁵M (25°C, pH7.1), 2.4×10⁻⁴M (37°C, pH7.1)(Acetoacetate)

8.4×10⁻⁶M (25°C, pH7.1), 1.5×10⁻⁵M (37°C, pH7.1)(NADH)

Inhibitors: PCMB, MIA, IAA, Ag⁺, Hg⁺⁺, SDS, DAC

Optimum pH: 8.3

Optimum temperature: 55°C

pH Stability: pH 5.0-8.5 (25°C, 20hr)

Thermal stability: below 40°C (pH 6.5, 15min)

Applications

This enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.

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