Tribioscience Creatinine deiminase, Enzyme Activity (TBP0011)

Research Use Only. Not intended for human use.

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 10U/mg-solid or more (containing approx. 30% of stabilizer)

Contaminants: Creatinine amidohydrolase ≤1.0×10⁻²%;

Creatine amidinohydrolase ≤1.0×10⁻²%;

Urease ≤1.0×10⁻²%;

NADH oxidase ≤1.0×10⁻²%;

NH4⁺ ≤1.0×10⁻²% µg/u

Stabilizer: Mannitol

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 260,000

Isoelectric point: 4.4

Michaelis constant: 3.5×10⁻³M (Creatinine)

Structure: 6 subunits per enzyme molecule

Inhibitors: Ag⁺, Hg⁺⁺, o-phenanthroline,monoiodoacetate

Optimum pH: 8.5-9.5

Optimum temperature: 65-75℃

pH Stability: pH 7.0-11.0 (30℃, 20hr)

Thermal stability: below 65℃ (pH 7.5, 1hr)

Applications

This enzyme is useful for enzymatic determination of creatinine when coupled with glutamate dehydrogenase in clinical analysis.

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