Product Details
Formal Name: 5-Carboxyfluorescein N-succinimidyl ester
Alternate Names: 5-Carboxyfluorescein NHS ester; 5-FAM-SE
Molecular Formula: C25H15NO9
Formula Weight: 473.3
CAS Number: 92557-80-7
Purity: >97%
Formulation: Powder
Solubility: Soluble in DMSO (35 mg/ml)
Storage: -20°C
Stability: ≥ 2 years.
Applications
Cellular proliferation probe
Functions
Fluorophore that can diffuse into cells and covalently react with intracellular proteins via its activated carboxy group and thus be retained in the cell for long periods of time. CFSE fluorescence can be used to analyze cell proliferation, track cell migration and in applications such as flow cytometry. Excitation/emission max: 491 and 518 nm.
Application Procedures
First dissolved in DMSO (35 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 3 months.