Tribioscience Beta-N-Acetylglucosaminidase (Beta-NAG) Activity Fluorometric Assay (TBS2105F)

Tribioscience Beta-N-Acetylglucosaminidase (Beta-NAG) Activity Fluorometric Assay (TBS2105F)

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β-N-Acetylglucosaminidase (β-NAG, β-Hexosaminidase, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidneys, liver, lungs, and mast cells. Elevated enzyme levels are associated with many disorders, such as Tay-Sachs and Sandhoff disease, inflammation, and abnormal immune responses.

The β-N-Acetylglucosaminidase Activity Fluorometric Assay provides a simple, accurate, and sensitive method for monitoring N-acetylglucosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a non-fluorescent substrate 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUAG) to release 4-Methylumbelliferone (4-MU) which can be measured at an excitation wavelength of 365 nm and an emission wavelength 445 nm.

Applications

This kit is used for the determination of hexosaminidase activity in biological samples.

Key features

• Fast and sensitive: Linear detection range (20 µL sample) for a 30 minutes reaction at 37˚C.
• High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
  

Storage Conditions

The kit is shipped on ice and should be stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

For Research Use only

Preparation

Standard Curve