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Description: The DYKDDDDK-Tag, also known as the FLAG tag, is a short peptide sequence used for protein tagging and purification. It consists of eight amino acids: DYKDDDDK, and is designed for immunopurification chromatography. The DYKDDDDK-Tag can be used in various expression systems and can be applied as a single peptide or in tandem repeats to enhance epitope recognition .
Applications:
- Elisa
- Flow cytometry
- Immunoblotting
- Immunoprecipitation
- Immunocytochemistry
- Immunofluorescence
- Chromatin immunoprecipitation
- Electron microscopy
- Supershift assays
- Protein purification if conjugated with agarose or magnetic beads
- Isotype controls
Molecular Weight: 150 kDa
Host: Mouse
Isotype: Mouse IgG1 kappa
Concentration: 5.0 mg/ml
Purity: >95%
Formulation: PBS, pH 7.0, no stabilizers or preservatives.
Endotoxin: < 1EU/mg
Clonality: Monoclonal
Sterility: 0.2 µm filtered
Storage: 4°C stable for up to one month. Store at -20°C for long-term.