Tribioscience’s Alkaline Phosphatase Activity Fluorometric Assay Kit provides a highly sensitive and convenient way for direct detection of ALP activity in different biological samples. In the assay, ALP removes the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (4-MUP) substrate producing an intense fluorescent signal (Ex/Em = 360 nm/450 nm). The kit is an ultra-sensitive, HTS-ready assay that is more sensitive than colorimetric methods. The assay is suited for both research and drug discovery.
Applications
Direct Assays: Acid phosphatase in serum, plasma, urine, and other bio-samples.
Key Features
- Flexible: Suitable for colorimetric assay.
- Accurate: Use 50 µL samples. Detection ranges 0.3-80 U/L in 96-well plate for fluorometric assay.
- Simple and high-throughput: Just load-incubate-Read. The kit can be used as a robust method.
- Time-saving: 20-30 minutes.
For Research Use only
Standard Curve Preparations (Fig. 1)
Typical Standard Curve (Fig. 2)
