Trypsin-EDTA solutions are commonly used to detach adherent cells from culture surfaces. Various concentrations of trypsin-EDTA are available, and their use depends primarily on the cell type and the age of the culture to dislodge cells from the substrate. The general recommended working concentration is 0.05% Trypsin for cell detachment.
0.05% Trypsin-EDTA is a ready-to-use solution (1x) without additional dilution.