Strep-Tag II Monoclonal Antibody
SKU: E-AB-48009-200
Strep-Tag II Monoclonal Antibody
| SKU # | E-AB-48009 |
| Reactivity | All |
| Host | Mouse |
| Applications | WB |
Product Details
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | All |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin. |
| Abbre | Strep-Tag II |
| Synonyms | Strep II, Strep Tag II |
| Calculated MW | 45 kDa |
| Observed MW | 45 kDa
|
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 4H10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-2000 |
Background
The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution.