Recombinant Swine IFN gamma protein(N-His)(active)
SKU: PKSS000012-20
Recombinant Swine IFN gamma protein(N-His)(active)
| SKU # | PKSS000012 |
| Expression Host | E.coli |
Description
| Synonyms | IFN-gamma, IFNG |
| Species | Porcine |
| Expression Host | E.coli |
| Sequence | Gln 24-Lys 166 |
| Accession | P17803 |
| Calculated Molecular Weight | 17.7 kDa |
| Observed Molecular Weight | 15 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to protect PK15 cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is <40 pg/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them; leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens; mitogens; or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2; FGF-basic; and EGF.