Recombinant Rat CD73/NT5E Protein (His Tag)
SKU: PKSR030462-50
Recombinant Rat CD73/NT5E Protein (His Tag)
| SKU # | PKSR030462 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 5'-Nucleotidase, CD73, E5NT, NT5E, Nucleotidase, ecto-5'-nucleotidase |
| Species | Rat |
| Expression Host | HEK293 Cells |
| Sequence | Trp29-Lys549 |
| Accession | P21588 |
| Calculated Molecular Weight | 59.0 kDa |
| Observed Molecular Weight | 70-90 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
Background
CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.