Recombinant Mouse TGFBR2 Protein (Fc Tag)
SKU: PKSM041170-50
Recombinant Mouse TGFBR2 Protein (Fc Tag)
| SKU # | PKSM041170 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | TGF-beta receptor type II, TGF-beta receptor type-2, TGF-beta type II receptor, TGFR-2, TbetaR-II, Tgfbr2, Transforming growth factor-beta receptor type II |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Ile24-Asp159 |
| Accession | Q62312-2 |
| Calculated Molecular Weight | 42.3 kDa |
| Observed Molecular Weight | 55-65 kDa |
| Tag | C-Fc |
| Bio-activity | Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells.The ED50 for this effect is 69.07 ng/ml in the presence of 1ng/ml of recombinant human TGF-beta 1. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Transforming growth factor-β (TGF-β) is an essential regulator in the processes of development, cell proliferation, and extracellular matrix deposition. TGF-β regulates cellular processes by binding to three high-affinity cell surface receptors: TGF-β receptor type I (TGF-β-RI), TGF-β receptor type II (TGF-β-RII), and TGF-ββ receptor type III (TGF-β-RIII). TGF-β RII is consists of a C-terminal protein kinase domain and an N-terminal ectodomain and belongs to transforming growth factor-beta (TGF-β) receptor subfamily. TGF-β RII has a protein kinase domain which can form a heterodimeric complex with another receptor protein and bind TGF-beta. This receptor/ligand complex phosphorylates protein will enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.