Recombinant Mouse SLAMF5/CD84 Protein (His Tag)(Active)
SKU: PKSM040499-100
Recombinant Mouse SLAMF5/CD84 Protein (His Tag)(Active)
| SKU # | PKSM040499 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD84, CD84 antigen, CD84 molecule, Cd84, Leukocyte differentiation antigen CD84, Ly-9B, SLAM family member 5, SLAMF5, Signaling lymphocytic activation molecule 5 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Val 221 |
| Accession | Q18PI6-1 |
| Calculated Molecular Weight | 24 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.