Recombinant Mouse SCF/c-Kit Ligand Protein (His Tag)
SKU: PKSM041311-50
Recombinant Mouse SCF/c-Kit Ligand Protein (His Tag)
| SKU # | PKSM041311 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | FPH2, Hematopoietic growth factor KL, KIT ligand, KITLG, KL-1, Kitl, MGF, MGFSHEP7, Mast cell growth factor, SCF, SFc-Kit ligand, SLF, Stem cell factor, steel factor |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Lys26-Ala189 |
| Accession | P20826 |
| Calculated Molecular Weight | 19.3 kDa |
| Observed Molecular Weight | 19-35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.