Recombinant Mouse β-NGF/Beta-NGF Protein (Active)
SKU: PKSM040709-20
Recombinant Mouse β-NGF/Beta-NGF Protein (Active)
| SKU # | PKSM040709 |
| Expression Host | CHO Stable Cells |
Description
| Synonyms | Ngfb |
| Species | Mouse |
| Expression Host | CHO Stable Cells |
| Sequence | Ser 122-Gly 241 |
| Accession | NP_001106168.1 |
| Calculated Molecular Weight | 13.5 kDa |
| Observed Molecular Weight | 13.5 kDa |
| Tag | None |
| Bio-activity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL. |
Properties
| Purity | > 96 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.