Recombinant Mouse Kininogen-1/KNG1 Protein (His Tag)(Active)
SKU: PKSM040662-50
Recombinant Mouse Kininogen-1/KNG1 Protein (His Tag)(Active)
| SKU # | PKSM040662 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Kng |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Glu 21-Ser 480 |
| Accession | NP_001095882.1 |
| Calculated Molecular Weight | 52.5 kDa |
| Observed Molecular Weight | 70-80 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (R&D Systems, Catalog # ES009). The IC50 value is < 5 nM. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20mM Tris, 100mM NaCl, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
GPNMB belongs to the PMEL / NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 ( HGFIN ), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin as a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.