Recombinant Mouse Interleukin-33/IL-33 Protein (Active)
SKU: PKSM041090-100
Recombinant Mouse Interleukin-33/IL-33 Protein (Active)
| SKU # | PKSM041090 |
| Expression Host | E.coli |
Description
| Synonyms | DVS, NF-HEV and IL- 1F, Interleukin-1 family member, C9orf, C9orf26, DVS27, Interleukin-1 family member 11, NF-HEV and IL- 1F11, NKHEV, IL33, Interleukin-33, IL-33, Interleukin 33, IL1F11, NFEHEV, NF-HEV |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Ser109-Ile266 |
| Accession | Q8BVZ5 |
| Calculated Molecular Weight | 18.5 kDa |
| Observed Molecular Weight | 17-25 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 107 IU/mg. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.