Recombinant Mouse ALCAM/CD166 Protein (His & Fc Tag)(Active)
SKU: PKSM040924-100
Recombinant Mouse ALCAM/CD166 Protein (His & Fc Tag)(Active)
| SKU # | PKSM040924 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | AI853494, BEN, CD166, DM-GRASP, MuSC, SC1 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Lys 527 |
| Accession | NP_033785.1 |
| Calculated Molecular Weight | 84.2 kDa |
| Observed Molecular Weight | 105-110 kDa & 210 kDa |
| Tag | C-His-Fc |
| Bio-activity | Immobilized mouse CD6-His at 10 μg/ml (100 μl/well) can bind mouse ALCAM-Fc2h, The EC50 of mouse ALCAM-Fc2h is 0.12-0.28 μg/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.