Recombinant Klebsiella pneumoniae NEO Protein
SKU: PKSQ050062-50
Recombinant Klebsiella pneumoniae NEO Protein
| SKU # | PKSQ050062 |
| Expression Host | E.coli |
Description
| Synonyms | APH(3')-II, APH(3')II, Aminoglycoside 3'-phosphotransferase, Kanamycin kinase type II, Neomycin-kanamycin phosphotransferase type II, neo |
| Species | Klebsiella pneumoniae |
| Expression Host | E.coli |
| Sequence | Met1-Phe264 |
| Accession | P00552 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 26-30 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween 80, PH8.0. |
| Reconstitution | Not Applicable |
Background
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.