Recombinant Human PVRIG (C-mFc) Expression Host HEK293 Cells
SKU: PKSH034019-50
Recombinant Human PVRIG (C-mFc) Expression Host HEK293 Cells
| SKU # | PKSH034019 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | C7orf15, C7orf15MGC138295, CD112R, MGC104322, MGC138297, MGC2463, PVRIG, transmembrane protein PVRIG |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Thr41-Asp171 |
| Accession | Q6DKI7 |
| Calculated Molecular Weight | 40.1 kDa |
| Observed Molecular Weight | 45-55 kDa |
| Tag | C-mFc |
| Bio-activity | Loaded Biotinylated Human Nectin-2-His on His Biosensor, can bind Human PVRIG with an affinity constant of 84.68 nM as determined in BLI assay. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells. PVRIG may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.