Recombinant Human Fibronectin/FN Protein (His & Avi Tag)
SKU: PKSH033677-100
Recombinant Human Fibronectin/FN Protein (His & Avi Tag)
| SKU # | PKSH033677 |
| Expression Host | E.coli |
Description
| Synonyms | CIG, ED-B, FINC, FN, FN1, FNZ, Fibronectin, GFND, GFND2, LETS, MSF |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Glu1266-Thr1356 |
| Accession | P02751-15 |
| Calculated Molecular Weight | 13.4 kDa |
| Observed Molecular Weight | 15 kDa |
| Tag | N-His-Avi |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Similar to integrins; fibronectin binds extracellular matrix components such as collagen; fibrin; and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion; growth; migration; and differentiation; and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression; degradation; and organization has been associated with a number of pathologies; including cancer and fibrosis. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer; named superfibronectin; exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth; angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.