Recombinant Human APRIL protein(N-His)(active)
SKU: PKSH034120-100
Recombinant Human APRIL protein(N-His)(active)
| SKU # | PKSH034120 |
| Expression Host | E.coli |
Description
| Synonyms | CD256, TALL-2, TALL2, TNFSF13, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala 1-Leu 146 |
| Accession | AAQ91388 |
| Calculated Molecular Weight | 17.3 kDa |
| Observed Molecular Weight | 18 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to induce cell death in Jurkat cells. The ED50 for this effect is 2.6-4.0 μg/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS with 0.1% sarkosyl,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
APRIL (a proliferation-inducingligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely ofa single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signalthrough the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfateproteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers withBAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells andalso promotes T cell-dependenthumoral responses. APRIL levels are elevated inthe serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratingneutrophils.