Recombinant Human ACE2 Protein (mFc Tag) Store at < -20°C
SKU: PKSR030494-50
Recombinant Human ACE2 Protein (mFc Tag) Store at < -20°C
| SKU # | PKSR030494 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ACE-Related Carboxypeptidase, ACE2, ACEH, Angiotensin-Converting Enzyme 2, Angiotensin-Converting Enzyme Homolog, Metalloprotease MPROT15 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln18-Ser740 |
| Accession | Q9BYF1 |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 110-140 kDa |
| Tag | C-mFc |
| Bio-activity | Immobilized 2019-nCoV S1 Protein-Fc at 10μg/ml (100 μl/well) can bind Human ACE-2-mFc. The ED50 of Recombinant Human ACE-2-mFc is 0.58 ug/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 300mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.4. |
| Reconstitution | Not Applicable |
Background
Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.