Recombinant HCV (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag)
SKU: PKSV030179-100
Recombinant HCV (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag)
| SKU # | PKSV030179 |
| Expression Host | E.coli |
Description
| Synonyms | HCV, NS3 Protein |
| Species | HCV |
| Expression Host | E.coli |
| Sequence | Thr1356-Thr1459 |
| Accession | NP_803144.1 |
| Calculated Molecular Weight | 37.9 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 50 mM Tris, 500 mM NaCl. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS.