Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
| SKU # | E-BC-K014 |
| Detection Instrument | Semi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate reader |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | NEFA |
| Sample Type | Serum, plasma, tissue homogenate, cells, cell supernatant |
| Detection Range | 0.01-3.0 mmol/L |
| Detection Method | Colorimetric method |
| Assay type |
Quantitative |
| Other instruments required | Normal saline (0.9% NaCl) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Detection Principle
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.