NGEF Polyclonal Antibody Reactivity Human
SKU: E-AB-52771-200
NGEF Polyclonal Antibody Reactivity Human
| SKU # | E-AB-52771 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human NGEF |
| Abbre | NGEF |
| Synonyms | neuronal, ARHGEF27, EPHEXIN, Eph receptor-interacting exchange protein, Eph-interacting exchange protein, Ephexin-1, Guanine nucleotide exchange factor, NGEF, Neuronal guanine nucleotide exchange factor, Ngef |
| Swissprot | |
| Calculated MW | 82 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Membrane. Cell projection>growth cone. Associated with membranes. Localizes to axonal growth cones. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Neuroscience, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:30-1:150 |
Background
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).