MSE PRO Sheep bEP(Beta-Endorphin) ELISA Kit
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MSE PRO™ Sheep bEP(Beta-Endorphin) ELISA Kit
Specifications:
| Product name | Sheep bEP(Beta-Endorphin) ELISA Kit |
| Reactivity | Sheep |
| Alternative Names | Beta EP; b-EP |
| Assay Type | Competitive Inhibition |
| Sensitivity | 6.15 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Signal transduction; Endocrinology; Neuro science; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep bEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep bEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep bEP in the samples is then determined by comparing the OD of the samples to the standard curve. |