MSE PRO Rat TREM1(Triggering Receptor Expressed On Myeloid Cells 1) ELISA Kit
SKU: EK0064592-96T
MSE PRO™ Rat TREM1(Triggering Receptor Expressed On Myeloid Cells 1) ELISA Kit
Specifications:
| Product name | Rat TREM1(Triggering Receptor Expressed On Myeloid Cells 1) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | CD354; Triggering Receptor Expressed On Monocytes-1 |
| Assay Type | Sandwich |
| Sensitivity | 6.7 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cytokine; Infection immunity |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TREM1 in the samples is then determined by comparing the OD of the samples to the standard curve. |