MSE PRO Rat PRM1(Protamine 1) ELISA Kit
SKU: EK0064531-96T
MSE PRO™ Rat PRM1(Protamine 1) ELISA Kit
Specifications:
| Product name | Rat PRM1(Protamine 1) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | CT94.1; Cancer/Testis Antigen Family 94,Member 1; Sperm protamine P1; Cysteine-rich protamine |
| Assay Type | Sandwich |
| Sensitivity | 0.056 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Reproductive science |
| Uniprot ID | P10118 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRM1 in the samples is then determined by comparing the OD of the samples to the standard curve. |