MSE PRO Rat ACSL4(Acyl-CoA Synthetase Long Chain Family Member 4) ELISA Kit
SKU: EK0064832-96T
MSE PRO™ Rat ACSL4(Acyl-CoA Synthetase Long Chain Family Member 4) ELISA Kit
Specifications:
| Product name | Rat ACSL4(Acyl-CoA Synthetase Long Chain Family Member 4) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | ACS4; FACL4; LACS4; MRX63; MRX68; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Mental Retardation, X-Linked 63; Lignoceroyl-CoA Synthase |
| Assay Type | Sandwich |
| Sensitivity | 0.061 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACSL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACSL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACSL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACSL4 in the samples is then determined by comparing the OD of the samples to the standard curve. |