MSE PRO PGE2(Prostaglandin E2) ELISA Kit
SKU: EK0064253-48T
MSE PRO™ PGE2 (Prostaglandin E2) ELISA Kit
Specifications:
| Product name | PGE2 (Prostaglandin E2) ELISA Kit |
| Reactivity | General |
| Alternative Names | PG-E2 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 10.2 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample type | serum, plasma and other biological fluids |
| Assay length | 2h |
| Research Area | Metabolic pathway; Infection immunity; Endocrinology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve. |