MSE PRO PD1(Protectin D1) ELISA Kit
SKU: EK0064930-96T
MSE PRO™ PD1(Protectin D1) ELISA Kit
Specifications:
| Product name | PD1(Protectin D1) ELISA Kit |
| Reactivity | General |
| Alternative Names | Neuroprotectin D1; NPD1 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.072 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Immunology & Inflammation |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PD1 in the samples is then determined by comparing the OD of the samples to the standard curve. |