MSE PRO Mouse SHH(Sonic Hedgehog Homolog) ELISA Kit
SKU: EK0064820-96T
MSE PRO™ Mouse SHH(Sonic Hedgehog Homolog) ELISA Kit
Specifications:
| Product name | Mouse SHH(Sonic Hedgehog Homolog) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N |
| Assay Type | Sandwich |
| Sensitivity | 5.51 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SHH in the samples is then determined by comparing the OD of the samples to the standard curve. |