MSE PRO Mouse PKCβ1(Protein Kinase C Beta 1) ELISA Kit
SKU: EK0064917-96T
MSE PRO™ Mouse PKCβ1(Protein Kinase C Beta 1) ELISA Kit
Specifications:
| Product name | Mouse PKCβ1(Protein Kinase C Beta 1) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | PKC-B1; PRKCB; PRKCB1 |
| Assay Type | Sandwich |
| Sensitivity | 0.18 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction; Enzyme & Kinase; Metabolic pathway; Apoptosis; Tumor immunity |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PKCβ1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PKCβ1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PKCβ1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PKCβ1 in the samples is then determined by comparing the OD of the samples to the standard curve. |