MSE PRO Mouse CD80(T-lymphocyte Activation Antigen CD80) ELISA Kit
SKU: EK0064938-96T
MSE PRO™ Mouse CD80(T-lymphocyte Activation Antigen CD80) ELISA Kit
Specifications:
| Product name | Mouse CD80(T-lymphocyte Activation Antigen CD80) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1 |
| Assay Type | Sandwich |
| Sensitivity | 6.05 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule; Autoimmunity; |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD80. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD80. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD80, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD80 in the samples is then determined by comparing the OD of the samples to the standard curve. |