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MSE PRO Human TOMM22(Mitochondrial Import Receptor Subunit TOM22 Homolog) ELISA Kit

SKU: EK0064708-96T

  • $ 45795
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MSE PRO™ Human TOMM22(Mitochondrial Import Receptor Subunit TOM22 Homolog) ELISA Kit

Specifications:

Product name Human TOMM22(Mitochondrial Import Receptor Subunit TOM22 Homolog) ELISA Kit
Reactivity Human
Alternative Names hTom22; TOM22; 1C9-2; Translocase of outer membrane 22 kDa subunit homolog
Assay Type Sandwich
Sensitivity 0.11 ng/mL
Standard 20 ng/mL
Detection Range 0.32-20 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Assay Length 3.5h
Research Area Enzyme & Kinase
Uniprot ID Q9NS69
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TOMM22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TOMM22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TOMM22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TOMM22 in the samples is then determined by comparing the OD of the samples to the standard curve.