MSE PRO Human OXB(Orexin B) ELISA Kit
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MSE PRO™ Human OXB(Orexin B) ELISA Kit
Specifications:
| Product name | Human OXB(Orexin B) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Hypocretin-2 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.11 ng/mL |
| Standard | 25 ng/mL |
| Detection Range | 0.4-25 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Neuro science |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OXB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OXB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OXB in the samples is then determined by comparing the OD of the samples to the standard curve. |