MSE PRO Human NEB(Noradrenaline Bitartas) ELISA Kit
SKU: EK0064533-96T
MSE PRO™ Human NEB(Noradrenaline Bitartas) ELISA Kit
Specifications:
| Product name | Human NEB(Noradrenaline Bitartas) ELISA Kit |
| Reactivity | Human |
| Alternative Names | (R)-(−)-norepinephrine; (R)-4-(2-amino-1-hydroxyethyl)-1,2-benzenediol; (R)-noradrenaline |
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.1 ng/mL |
| Standard | 400 ng/mL |
| Detection Range | 6.25-400 ng/mL |
| Sample Type | Serum, plasma, other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal Transduction |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NEB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEB in the samples is then determined by comparing the OD of the samples to the standard curve. |