MSE PRO Human gABRa5(Gamma-Aminobutyric Acid A Receptor Alpha 5) ELISA Kit
SKU: EK0064847-96T
MSE PRO™ Human gABRa5(Gamma-Aminobutyric Acid A Receptor Alpha 5) ELISA Kit
Specifications:
| Product name | Human gABRa5(Gamma-Aminobutyric Acid A Receptor Alpha 5) ELISA Kit |
| Reactivity | Human |
| Alternative Names | GABA(A) receptor subunit alpha-5 |
| Assay Type | Sandwich |
| Sensitivity | 0.061 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Tissue homogenates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | CD & Adhesion molecule |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human gABRa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human gABRa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human gABRa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human gABRa5 in the samples is then determined by comparing the OD of the samples to the standard curve. |