MSE PRO Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit
SKU: EK0064556-96T
MSE PRO™ Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit
Specifications:
| Product name | Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CoII2-1 |
| Assay Type | Sandwich |
| Sensitivity | 18.2 pg/mL |
| Standard | 3000 pg/mL |
| Detection Range | 46.88-3000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Metabolic pathway |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CoII2-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CoII2-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CoII2-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CoII2-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |