MSE PRO Human AGE(Advanced Glycation End Product) ELISA Kit
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MSE PRO™ Human AGE(Advanced Glycation End Product) ELISA Kit
Specifications:
| Product name | Human AGE(Advanced Glycation End Product) ELISA Kit |
| Reactivity | Human |
| Alternative Names | AGEs; Advanced Glycation End Products |
| Assay Type | Competitive Inhibition |
| Sensitivity | 38.2 ng/mL |
| Standard | 8000 ng/mL |
| Detection Range | 125-8000 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway; Endocrinology; Cardiovascular biology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGE in the samples is then determined by comparing the OD of the samples to the standard curve. |