MSE PRO Horse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
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MSE PRO™ Horse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Specifications:
| Product name | Horse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit |
| Reactivity | Horse |
| Alternative Names | TBARS; Thiobarbituric Acid Reactive Substance |
| Assay Type | Competitive Inhibition |
| Sensitivity | 3.55 nmol/mL |
| Standard | 400 nmol/mL |
| Detection Range | 6.25-400 nmol/mL |
| Sample Type | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway; Hepatology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve. |