MSE PRO Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit

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SKU: EK0064476-96T
Regular price $ 319.95

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MSE PRO™ Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit

Specifications:

Product name Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit
Reactivity Horse
Alternative Names AOPP
Assay Type Competitive Inhibition
Sensitivity 49.7 pg/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type Serum, plasma and other biological fluids
Assay Length 2h
Research Area Tumor immunity
Test principle This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse AOPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.