MSE PRO Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit
SKU: EK0064476-96T
MSE PRO™ Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit
Specifications:
| Product name | Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit |
| Reactivity | Horse |
| Alternative Names | AOPP |
| Assay Type | Competitive Inhibition |
| Sensitivity | 49.7 pg/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Tumor immunity |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse AOPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse AOPP in the samples is then determined by comparing the OD of the samples to the standard curve. |