MSE PRO EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit
SKU: EK0064943-96T
MSE PRO™ EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit
Specifications:
| Product name | EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel Tertroxin |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.31 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Endocrinology; Autoimmunity; |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve. |