MSE PRO EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit
SKU: EK0064838-96T
MSE PRO™ EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit
Specifications:
| Product name | EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit |
| Reactivity | Human |
| Alternative Names | AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1 |
| Assay Type | Sandwich |
| Sensitivity | 37 pg/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Signal transduction |
| Uniprot ID | D4A523 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3, and the Human NLRP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NLRP3. After TMB substrate solution is added, only those wells that contain Human NLRP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |