MSE PRO EasyStep Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
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MSE PRO™ EasyStep Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
Specifications:
| Product name | EasyStep Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit |
| Reactivity | Human |
| Alternative Names | LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2 |
| Assay Type | Sandwich |
| Sensitivity | 5.6 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Infection immunity; Urology |
| Uniprot ID | P80188 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NGAL, and the Human NGAL standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NGAL. After TMB substrate solution is added, only those wells that contain Human NGAL and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve. |