MSE PRO EasyStep Human MIg(Monokine Induced By Interferon Gamma) ELISA Kit
SKU: EK0064773-96T
MSE PRO™ EasyStep Human MIg(Monokine Induced By Interferon Gamma) ELISA Kit
Specifications:
| Product name | EasyStep Human MIg(Monokine Induced By Interferon Gamma) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CXCL9; CMK; Humig; MI-G; SCYB9; Crg-10; Chemokine (C-X-C motif) ligand 9; Small-inducible cytokine B9; Gamma-interferon-induced monokine |
| Assay Type | Sandwich |
| Sensitivity | 15.7 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.2-5000 pg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Cytokine; Infection immunity |
| Uniprot ID | Q07325 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIg, and the Human MIg standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MIg. After TMB substrate solution is added, only those wells that contain Human MIg and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIg in the samples is then determined by comparing the OD of the samples to the standard curve. |