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MSE PRO EasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit

SKU: EK0064821-96T

  • $ 45795
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MSE PRO™ EasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit

Specifications:

Product name EasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit
Reactivity Human
Alternative Names C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Assay Type Sandwich
Sensitivity 21 pg/mL
Standard 5000 pg/mL
Detection Range 78.13-5000 pg/mL
Sample Type Serum, plasma
Assay Length 1.5h
Research Area Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology
Uniprot ID P02741
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRP, and the Human CRP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CRP. After TMB substrate solution is added, only those wells that contain Human CRP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.