MSE PRO EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit
SKU: EK0064538-96T
MSE PRO™ EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit
Specifications:
| Product name | EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit |
| Reactivity | Human |
| Alternative Names | GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32 |
| Assay Type | Sandwich |
| Sensitivity | 6.8 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Cardiovascular biology; Neuro science; Hormone metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP. After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve. |