MSE PRO EasyStep Human AMH(Anti-Mullerian Hormone) ELISA Kit
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MSE PRO™ EasyStep Human AMH(Anti-Mullerian Hormone) ELISA Kit
Specifications:
| Product name | EasyStep Human AMH(Anti-Mullerian Hormone) ELISA Kit |
| Reactivity | Human |
| Alternative Names | MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance |
| Assay Type | Sandwich |
| Sensitivity | 0.028 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Endocrinology; Reproductive science ;Hormone metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AMH, and the Human AMH standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human AMH. After TMB substrate solution is added, only those wells that contain Human AMH and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AMH in the samples is then determined by comparing the OD of the samples to the standard curve. |