MSE PRO 8-iso-PGF2α(8-isoprostane) ELISA Kit
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MSE PRO™ Mouse 8-iso-PGF2α(8-isoprostane) ELISA Kit
Specifications:
| Product name | Mouse 8-iso-PGF2α(8-isoprostane) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | 8-isoprostane; 8-iso-PGF2α |
| Assay Type | Competitive Inhibition |
| Sensitivity | 9.252 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.625-1000 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Endocrinology; Pulmonology; Hepatology; Hormone metabolism |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve. |